Phytochemistry and Pharmacology of Tinospora cordifolia: A Review

Tiwari, Nayak, Prusty, and Sahu: Phytochemistry and Pharmacology of Tinospora cordifolia: A Review



T. cordifolia commonly called as Guduchi is a natural herbal shrub that belongs to the moonseed family Menispermaceae. This plant is useful in treatment of several diseases like jaundice, skin diseases, gout, diabetes etc. which has been established in the history of traditional medicine practices.1 In this perspective, guduchi is considered to be a nectar plant and has been called as amrita in Sanskrit in recognition of its detoxifying, rejuvenating, and immune boosting properties.2 In modern medicine, the herb has been evaluated and studied more profoundly and most recently the drug is implemented to mitigate the negative effects of chemotherapy. The review focuses on the phytochemical study, pharmacological aspects and its scope for the scientific investigation for further progress in the field of traditional medicine.


We have exhaustibly reviewed many published literature on recent developments in research of T. cordifolia, including original articles and papers as secondary data from various search engine such as Pubmed, Pubmed Central Databases, Google Scholar, Crossref, WorldCat, Harvard library, Mendeley, Scilit, Cite factor, Shodhganga, Science Central, AYUSH Research Portal, Open J-Gate, Biblioteca were taken into consideration for the report. Medical advancements and the effects of T. cordifolia observed with different experiments were collected for the review purpose.


Guduchi is a large extensively spreading glabrous, perennial deciduous twine with succulent stems and papery bark which is widely found in India, Myanmar and Sri Lanka. It is native to the tropical region of India ascending to an altitude of 500 metres in the temperature range of 25 to 45 C.3 The leaves are simple, heart shaped and dark bright green in colour. Further, it is alternate, estipulate, entire and the lamina is broadly ovate 10-12 cm long and 8-15 cm broad showing multicoated reticulate venation.4 The surface of the stems appears to be closely studded with warty tubercles and the surface skin is longitudinally fissured, having 3-5cm length and 3-8mm in diameter.5,6 Bark is succulent, with deep clefts spotted and large rosette-like lenticels. The colour of bark is creamy white or grey. Long thread-like aerial roots arise from the branches. Branches are long and dirty white or light greyish brown in colour.7 Flowers are unisexual, small, and greenish yellow on auxiliary and terminal racemes. Male flowers are clustered whereas female flowers usually exist in a solitary inflorescence. A flower has six sepals, free in two series of three each and six petals which are free and smaller than sepals, ovate and membranous.8 Flowers grow during the summer (March to June) while fruits develop during the winter (November). Fruits are orange–red in colour, fleshy, aggregate of 1-3 ovoid, smooth, droplets on thick stalk with a sub terminal style scars.9 Curved seed and embryo have been described in guduchi.10 Hence, the family is named as moonseed family. Moreover, the endocarp is variously ornamented (Figure 1).


Ayurveda a 5,000-year-old system of medicine, names three elemental substance such as Kapha, Vata and Pitta rooted in Indian scriptures known as The Vedas. As per Ayurvedic text viz: Ashtang Hridaya and Sushrut, Charak and other treaties like Bhava Prakash and Dhanvantri Nighantu T. cordifolia named as : Amara, Amritvalli, Chinmarrhuha, Chinnodebha and Vatsadani etc, and most commonly recognised as Guduchi or Amrita.11-15 In Sushurta Samhita, under Tikta-SakaVarga, it is traditionally claimed for the treatment of several diseases like Svasa (asthma), Maha Jvara (fever), Aruci (anorexia) and kustha (leprosy).14 In context of Ashtang Hridaya and Charak Samhita, there is also a great evidence for the treatment of different diseases like Jvara (fever), Vat Rakta (gout) and Kamala (jaundice).13,15 In Bhavya Prakash, it is considered as diuretic, astringent, bitter tonic and potential curative and aphrodisiac against jaundice, diabetes, chronic diarrhea, dysentery and skin infections.16 In Dhanvantri Nighantu, it has been depicted for treatment of bleeding piles, curing itching, erysipelas and promoting longevity.17 Additionally, guduchi has been shown as: Deepanam (kindles digestive fire), Laghu (light), Dhatukrit (builds the seven bodily tissues), Chakshushyam (good for the eyes), Bayasthaapankarakam (maintains youthfulness and longevity) and Medhayam (rejuvenating for the mind).18

Figure 1

A: Whole plant, B: Stem part of the plant.

Guduchi has been considered by European practitioners in India as a major source of medicament like tonic, diuretic and antiperiodic and further it was comprised in Bengal Pharmacopoeia of 1868.19 According to Ayurvedic literature T. cordifolia is a major constituent of formulations used for the treatment of several disease such as dyspepsia, urinary related diseases debility and fever. Some of the imperative formulations prepared from T. cordifolia are: Guduchi taila, Sanjivani vati, Kanta-Kari avaleha, Guduchyadi churna, Chyavnaprasha, Guduchu ghrita, Guduchi satva, Brihat guduchi taila, Amrita guggulu, amritashtaka churna and many more.

T. cordifolia is the mostly used herbs of Ayurvedic medicine that, has been widely used by folks and tribal as a remedial herb for the treatment of various diseases. T. cordifolia is highly valuable in Ayurveda for its numerous medicinal properties like rejuvenating, immune-boosting, anti-rheumatic and detoxifying properties. Medicinal properties of T. cordifolia is presently applied in modern medicine for treatment of cold and flu prevention, skin disorders, liver disorders, immune support, gout, arthritis and lately to overcome the adverse effects of chemotherapy.18 So, now it is clear that T. cordifolia is most important medicinal herb considered by the ancient rishis in Vedic times with a great potential (medicinal qualities) of curing number of diseases.


T. cordifolia (Guduchi) mainly consists of alkaloids, glycosides, steroids, aliphatic compounds, essential oils, mixture of fatty acid, calcium, phosphorous, protein and polysaccharides (Figure 2).20


Stem and root part of T. cordifolia contain alkaloids as active constituents. These are tembetarine, choline, magnoflorine, berberine, tinosporin, isocolumbin, palmetine, jatrorrhizine, aporphine alkaloids, tetrahydropalmatine which showed anti-cancer, anti-diabetes, anti-viral, anti-inflammatory, anti-psychiatric and immunomodulatory action.21-26

Further, whole plant of T. cordifolia contain furanolactone, diterpenoid Lactones, Cleodrane derivatives [(5R, 10R)-4R-8R-dihydroxy-cleroda-13(16), 14-dieno-17, 12S:18, 1S-dilactone], columbin tinosporides, tinosporin, jateorine. They showed biological actions such as Vasorelaxant, anti-inflammatory, anti-microbial, anti-hypertensive and anti-viral.27-31 Shoot part of T. cordifolia contains Steroids (Β-sitosterol, δ-sitosterol, 20 β-hydroxyecdysone, giloinsterol, Makisterone A, Ecdysterone). They are effective in glucocorticoid induced osteoporosis in early inflammatory arthritis. They induce cell cycle arrest in G2/M phase and inhibits TNF-α, IL-1 β, IL-6 and COX-2 and apoptosis through c-Myc suppression.32-34

Figure 2

Active Compounds of T. Cordifolia.
Figure 3

Phytoactive Constituents of T. cordifolia.

Stem part of T. cordifolia contain Glycosides. Their active constituents are 18-norcleodrane glucoside, Tinocordifolioside, Cordioside, cordifolioside A, B, C, D and E, Furanoid diterpine glucoside, Syringin, pregnane glycoside Syringing-apiosylglycoside, palmatosides. They showed immunomodulation in Parkinson’s disease, dementia, motor and cognitive disorder, neurological disorders like ALS. They inhibit NF-k Band to show anti-cancer properties.35-41 Whole plant of T. cordifolia contain aliphatic compounds. The active constituents are Octacosanol, Nanocosan-15-one dichloromethane, Heptacosanol. They showed anti-nociceptive and anti-inflammatory activity. They also inhibit TNF-α from binding to the DNA and provide protection against 6-hydroxydopamine induced Parkinsonism in rats.42-44

Stem part of T. cordifolia contain Sesquiterpenoids and Tinocordifolin which exhibits an antiseptic activity.45 The other parts of T. cordifolia contain active constituents such as Jatrorrhizine, Tinosporic acid, 3, (a, 4-di hydroxy-3-methoxy-benzyl)-4-(4-hydroxy-3-methoxy-benzyl) tetrahydrofuran, N-trans-feruloyltyramine as diacetate, Giloin. They showed a protective effect against HIV (human immunodeficiency virus).46,47 The chief Phytoconstituents of T. cordifolia are diterpenoid furano lactone, cordifolide, cordifol, heptacosanol, tinosporide, β-sitosterol, tinosporine, clerodane furano diterpine, tinosporaside, and columbin respectively (Figure 3). Alkaloids such as magniflorine, Berberine, palmatine, non-glycoside gilonin gilosterol, tembertarine, choline and tinosporin has been reported from the stem part of the T. cordifolia.48


T. cordifolia has been recognised as most extensively used plant since ages in traditional system of medicine for its spasmolytic, allergen-free and anti-diabetic property. The plant significantly improves immune system. This plant possesses many useful properties. Its root part is known for its stress relieving and antimalarial properties while its stem is being used as bitter stomachic and diuretic. It stimulates biliary secretion, enrich the blood and cure jaundice. The major biological activities of T. cordifolia include the following.


Various experimental models of animal have been taken to show the anti-cancer activity of plant guduchi. The radio protective property is well characterized by this plant as it considerably increases the weight of various tissues as well as body weight. In addition to this, it also protects from the gamma radiation (sub-lethal range) radiated on the testes of mice (Swiss Albino). The cultured HeLa cells when exposed to different concentration of methylene chloride extracts of T. cordifolia such as 0,5,10,25,50,and 100 μg/ml ; it showed an increase in cell death or cell killing as compared to untreated cultured cell (control) in a dosedependent manner.49 A study has also reported that, the hydroalcoholic extract of roots (aerial) of T. cordifolia on exposure to the liver as well as extrahepatic organs of mice (Swiss Albino) at 50 and 100mg/kg body weight shows an increase in Glutathione (GSH) level and other metabolizing enzymes. In addition to this, there is a significant decrease in production of malonaldehyde (MLD) level representing a decrease in free radical formation providing an antioxidative state of cell.50

An exposure of hexane extract of guduchi on mice having Ehrlich ascites tumor shows an inhibition of the proliferation of these tumor cell (G1 phase) and simultaneously it enhances the expression of ‘Bax’ gene (pro-apoptic) leading to apoptosis principally brought by caspases.51 Guduchi has been reported to possess a potent anti-tumor activity through a two-stage skin carcinogenesis model conducted in mouse. It shows a decrease in papillary tumors, its weight and its occurrence while it also brings up the level of phase-II enzymes in the treatment group correspondingly.52

T. cordifolia exhibited an add-on effect when combined with γ-radiation on mice introduced with cultured Ehlrich cells by decrease in Glutathione (GSH) level causing oxidative damage to these cancerous cells.53 Guduchi extract in researcher’s skin cancer model has shown to prevent the degree of micronucleus production in bone marrow cell and hence, increase the survival time in mice. However, T. cordifolia in combination with cyclophosphamide drug exhibits a cumulative effect in tumor inhibitory rate and survival percentage respectively.54 An extract of the isolated active constituent palmatine of plant T. cordifolia clearly indicates the anti-cancer potential in a Dimethylbenzanthracene induced (DMBA) skin cancer model conducted in mice (Swiss Albino).55 T. cordifolia also possesses anti-neoplastic property as it has significant ability in treating the brain tumor in C-6 glioma cell by decreasing the proliferation and differentiation rate as reported.56

The anti-cancer activity of secondary metabolite (such as magnoflorine, palmatine, jatrorrhizine, yangambin etc.) isolated from guduchi were tested in different type of tumor cells and among them ‘palmatine’ and ‘yangambin’ reported to treat KB cells while tinocordiside for colon cancer cell and oral cancerous cell (KB) respectively.57 On the contrary, most of the chemotherapeutic agents are synthetic by nature and have a number of adverse as well as severe toxic effects which is very minimal in case of herb T. cordifolia. So, it can be considered as a ‘safe drug’ for treating cancer disease as far as patient health is concerned.


Guduchi have a potential ability to scavenge free radical and shows a protective effect by altering different hormone and mineral levels. T. cordifolia has reported to reverse the toxicity caused by aflatoxin in kidney (Swiss albino mice) where, it substantially elevates the hormone level (such as Glutathione) and enzyme activities (such as catalase, glutathione reductase); and decreases the reactive oxygen species (ROS). And this anti-toxin activity is primarily brought by the alkaloids of this plant.58 Lead nitrate toxicity in swiss albino mice shows a decreased value in erythrocyte and leucocyte count in blood serum.

However, the leaf and stem extract of guduchi works against these changes by overcoming the lead induced toxicity over haematological value.59 This herbal plant extract when given orally has also reported to counter the toxic effects caused by lead nitrate in mice (swiss albino) liver. The study shows a decrease in level of the enzymes like glutamic pyruvic transaminase (GPT) or alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and a rise in the enzyme responsible for scavenging free radical such as catalase.60 T. cordifolia has found its importance in overcoming cyclophosphamide induced toxicity by substantially elevating the level of lowered GSH content, cytokines and gradually declining inflammatory cytokines (Tumor necrosis factor) level in urinary-bladder and hepatic cell preventing the damage which confirms its anti-toxin activity.61


The compounds such as alkaloids, cardiac glycosides, saponins, flavonoids, tannins and steroids isolated from guduchi possess anti-diabetic property. Hence, it makes possible to have wide application in clinical as well as experimental study. Alkaloids from guduchi stated to possess the effect like insulin hormone and shows insulin mediated actions.26 Gestational Diabetes can increase the GSH content and other reactive species that can act as a threat to the mother as well as fetus. However, a study stated that when T. cordifolia has been given in daily diet to a diabetic-pregnant rat (streptozocin induced diabetes), it shows a protective effect by reducing the oxidative load thereby preventing the relative incidence of diseases and any sort of birth defect.62 In diabetic rat model, root extracts of guduchi attenuate the brain mediated lipid level and downregulates the blood glucose and urinary glucose level emphasizing its anti-diabetic and lipid lowering activity.63

The root extract of guduchi shows antihyperglycemic effect in alloxan induced diabetic model by decreasing its excess glucose level in urine as well as in blood to a range of normal.64 Medicinal herbal preparations like Ilogen-Excel, Hyponidd and Dihar consist of number of herbal plants including guduchi. When these preparations have been tested in diabetic rat models, it was seen that the anti-diabetic activity is solely due to T. cordifolia. The effects by Ilogen Excel reported to turn down the level of excess glucose in blood and enhance the insulin efficiency by increasing its amount in the systemic circulation. Hyponidd is reported to maintain the oxidative load by decreasing reactive species and reduced the glucose mediated haemoglobin count. ‘Dihar’ when tested for one and half month in streptozotocin induced diabetic model decreased the urea as well as creatinine amount in blood with subsequent increase in enzyme activities.65-67

The stem extract of T. cordifolia is reported to have anti-diabetic potential by enhancing the insulin efficiency through its secretion from beta pancreatic cell and promoting various anti-diabetic pathway such as inhibiting glucose formation by enhancing glycogenesis etc. thereby decreasing the endogenous glucose.68 Extract of guduchi in a clinical study is reported to inhibit the glucosidase enzyme which thereby decreases the post meal increased glucose level.69 Oral administration of leaf extracts of T. cordifolia has also found anti-diabetic potential when tested in diabetic rat model (streptozotocin induced diabetes) through different peripheral pathways such as glycogen storage, transportation of glucose and other mechanisms.70


Isolated chemical compounds such as cordifolioside A and syringin of guduchi are reported as immunomodulating agent in the clinical study.71 T. cordifolia stem alters the level of enzymes such as catalase and stimulates lymphocyte cells maintaining the immune strength, thus highlighting the immuno-protective role of this shrub.72 Macrophage cell when exposed to T. cordifolia extract, increases the production of different enzymes including ‘myeloperoxidase’ that enhances the anti-microbial action so as to protect the immunity.73 On the other hand, it also increases the phagocytic activity of macrophages. Additionally, it stimulates splenocytes and macrophages. Because of enhanced nitric oxide production signifying anti-tumor as well as immuno-protective activity.74 A clinical study stated that, T. cordifolia lotion causes a decline in the level of interleukin i.e. IL-1 and IL-6 in scabies animal model. It inhibits hyperkeratosis and infiltration of inflammatory cells into scabietic gash, showing its anti-scabies activity.75 Aqueous extract induces cellular mitosis, stimulates the production and activation of cytokine and immune effector cells.74

T. cordifolia is also able to increase the response of immune cell and neutrophil activity highlighting it as a potent agent for the prophylaxis of immune susceptible diseases.76 Compounds of guduchi including alkaloids, steroids, aliphatic compounds etc when tested preclinically in rat model has shown a potent immuno-protective activity.77 A polysaccharide compound obtained from T. cordifolia recognised as G1-4A enhances the proliferation and differentiation of immune cells i.e. T-cell and B-cell associated with the expression of the anti-apoptotic gene.78 The compound α-D-glucan obtained from TC has shown to maintain the body physiology by activating the cells of lymphocytes.79 Polymorphonuclear leucocyte (PMN) cells are important components of the host defense system. Extracts of T. cordifolia stimulated the PMN cells for phagocytosis.80 Oral administration of T. cordifolia alcoholic extract (100mg/kg) initiates an increase in foot pad thickness as well as in white blood cell (WBC) count and bone marrow cells indicating a stimulatory effect on haemopoetic system which shows a potent immunomodulatory action.81 A classical preparation of an aqueous extract of T. cordifolia named as ‘Ghana’ in Ayurveda when tested on the edema rat model, it reduced the edematogenic agents and thus has a potent immunostimulatory action.82


A study reported that silver nanoparticles synthesized from the stem of T. cordifolia possess good antibacterial activity against the bacteria Pseudomonas aeruginosa found in the patient suffering from burn injury.83 Various bacterial strains such as S.typhi, K.Pneumoniae, E.coli, Aeruginosa and other bacteria have been tested against extracts of T. cordifolia and showed potential anti-bacterial activity by either inhibiting their growth or mitigating the very existence of these bacteria.84,85,86 An active chemical compound that has been found from the stem of T. cordifolia as reported, found to be effective against bacteria like E.faecalis and B.subtilis and fungus like T. Simii and T.rubrum.87 A hydro alcoholic extract of T. cordifolia was effective in the mammary inflammation induced in bovine model by enhancing the activity of granulocyte. As mastitis is due to the infection of S. aureus, prevention of this inflammation showed the antimicrobial activity of this plant.47,88

The stem and leaves of this plant showed maximum inhibitory activity against the clinical urinary pathogens such as Klebsiella pneumoniae and Pseudomonas aeruginosa. Thus, it prevents the urinary tract infections.89 Carbapenamases being a hydrolytic enzyme hydrolyzes the β-lactam antibiotics and turns out to be ineffective. So, when the extract of this plant tested against penicillinase resistant β-lactam antibiotic (methicillin) and carbapenamase enzyme producing bacteria it shows high rate of inhibition as compared to the referral microbial strain.90 Guduchi has also found its importance by decreasing the resistance to different antibiotic therapy by the urinary pathogens and thus check the microbial infectivity.91


Various extracts of T. cordifolia exhibits an anti-oxidant potential by scavenging the free radicals and other reactive species respectively.92 T. cordifolia significantly reduces the regulation of lipid peroxidation process thereby decreasing the level of reactive free radical species in a diabetic rat model (alloxan induced diabetes) and up regulates antioxidant enzymes like catalase and glutathione indicating its anti-oxidant effects.65,93,94 A clinical research has reported that the extract shows antioxidant effect by raising the level of GSH and reducing the expression of inducible nitric oxide synthase gene, while it is also useful in treatment of cataract by inhibiting the enzyme aldol reductase.95,96 A study also suggests that TC bark extracts (ethanol) shows the higher free radical scavenging activity as well as the highest phenolic content compared to the methanol extracts.97

The plant derived polysaccharide compound named as ‘arabinogalactan’ shows a protection against free radicals in rat model indicating its antioxidant action.98 T. cordifolia is reported to modify the levels of different enzymatic system which then controls the production of these reactive species and thereby maintains the oxidative load by regulating the lipid peroxidation process and glutathione level.99 This plant also protects the mice from γ-radiation due to its anti-oxidant property100-101 by inhibiting the ferrous sulphate generated lipid peroxidation.102 In Ayurveda medicine, Pepticare a herbomineral formulation which includes T. cordifolia has also been reported to possess potent anti-oxidant effect in rat model.103


T. cordifolia has been evaluated to found its importance in treating HIV positive patients by decreasing the patient’s resistance to the retroviral regimen.104 The anti-HIV activity of T. cordifolia uncovers its application in managing the disease by increasing the CD4 T-cells count and decreasing eosinophil-(a type of WBC) count in HIV positive patients. T. cordifolia extract showed significantly enhanced phagocytic and intracellular bactericidal activity. T. cordifolia also stimulated peritoneal macrophage. Furthermore, T. cordifolia increases phagocytosis and intracellular killing property. T. cordifolia significantly stimulates B-lymphocytes, polymorph nuclear leucocytes and macrophages.101,105,106


An in vitro study suggests, that the alcoholic extract of guduchi is found to enhance the degree of proliferation and differentiation of the osteoblast cells of both human and rats. Over and above it also take part in the calcification process by producing minerals by these bone forming cell models regulating the bone mineralization.107 A steroid named ‘Beta-Ecdysone’ (Ecd) or 20-hydroxyecdysone isolated from T. cordifolia showed to promote the building of muscle tissue in mesenchymal stem cells model of mouse preventing the incidence of osteoporosis.108,100

In addition to the above, some of the other important therapeutic activity associated with T. cordifolia are briefly summarised in the following Table 1.

Table 1

Table Shows the Therapeutic activity of Tinospora cordifolia.

ActivityPart/ExtractAnimal Model/Cell LinesReference
Cardioprotective effectWhole plant/ Alcohol extractCalcium chloride administrated by intravenous infusion to produce arrhythmia in rats24
 Whole plant/ Ethanol 109
Antiulcer activityAqueous extractsAlbino rats using pylorus ligation induced ulcer. 
 Whole plant/Ethanol 109
Antidiarrheal activityAqueous extractCastor oil and Magnesium sulphate induced diarrhea in albino rats. 
Analgesic activityWhole plant/Ethanol extractHot plate and abdominal writhing method in albino rats.110
Aphrodisiac propertyAqueous and hydroalcoholic extractAdult albino rats of wistar strain.111
Immunomodulatory activityWhole plant/Aqueous extractSwiss male albino mice.112
Antidyslipidemic activityStem ExtractAlloxan induced diabetic male adult rats of charles foster strain.113
 Aerial parts/ 114
Neuroprotective effectEthanol extracts6-hydroxy dopamine lesion rat models of Parkinson’s disease. 
Anti-inflammatory activityStem/Aqueous extractCarrageenan induced paw edema model in rats.115
Gastroprotective activityWhole plantIndomethacin induced gastric ulcer in rats.116
Antioxidant activityWhole plant/Ethanol extractN-nitrosodiethylamine induced liver cancer in male wistar albino rats.117
Radio protective and Cytoprotective activityStem/Ethanol extract4 Gy-? radiation in albino mice and cyclophosphamide induced genotoxicity.118
Antifeedant activityWhole plant/ Chloroform ExtractMicroorganism used: Earias vitella, Plutella xylostella, Spodoptera litura.119
Ameliorative effectRoot/Ethanol extractMale swiss albino mice exposed to aflatoxin B1.24
Hepatoprotective activityWhole plant/ Aqueous ExtractBile duct ligation induced jaundice in rats.120
Nootropic effectWhole plant/Ethanol extractAmnesic rats using radial arm maze task performance and barnes maze test.121
Hypoglycemic activityStem/ Aqueous ExtractInsulin released effect was detected in vitro using rat pancreatic ß-cell lines.26
Antipsychotic activityAqueous and Ethanol extractAmphetamine challenged mice model.122
Antidepressant activityPetroleum ether extractSwiss albino mice and activity was evaluated using tail suspension test and forced swim test.123
Antiosteoporotic activityStem/Ethanol extractFemale sprague-dawley rats.124
Antineoplastic activityAerial parts/DCM extractMice transplanted with ehrlich ascites carcinoma.25
Antifertility effectStem/Methanol extractMale rats.125
Antiasthamatic activityStem/Hydroalcoholic ExtractMice were sensitized with intraperitoneal ovalbumin followed by intranasal ovalbumin in vivo asthma model.126
Antitumor activityAqueous alcoholic extractC6 glioma cells were used, extract reduced the cell proliferation in dose dependant manner.127
Allergic rhinitisAqueous extractDouble blind placebo-controlled trial.128
Diabetic neuropathyStem/aqueous extractStreptozotocin induced wistar albino diabetic rats and in vitro aldose reductase inhibition assay and in vivo results were analysed with Mann whitney Test. 
Antimalarial activityStem/ Ethanolic extractMicroorganism used Plasmodium berghei on white swiss mice models.130
Hepatocellular carcinomaAerial parts/ Ether extractDiethyl nitrosamine induced hepatocellular carcinoma in male wistar rats.130
Anticancer activityAqueous and Ethanolic extractIMR 32 human neuroblastoma cell lines as a model system.131
Antibacterial activityStem/ Aqueous and Ethanolic ExtractMicroorganisms used: E. coli, P. vulgaris, E. faecalis, S. typhi, S. aureus, S. marcesenses.132


T. cordifolia being a resourceful plant constitute innumerable biologically active compounds that have been reported to have a therapeutic potential. There are reports in pharmacological and clinical studies which validate the curative and remedial role of this plant to combat different ailments. The different bioactive compounds including alkaloids, steroids, glycosides, sesquiterpenoids etc found to have potential application especially as immunomodulator and anti-oxidant agent. The various studies that have been conducted on T. cordifolia reveals that it is an excellent drug and does not have any adverse or toxic effects till now. Overall, this review gives information about the classical anti-toxin, antidiabetic, anticancer, immunomodulatory, antioxidant, antimicrobial activity of T. cordifolia and can be used for further research investigations in development of novel drug.


HIV: Human Immunodeficiency Virus; GSH: Glutathione Stimulating Hormone; DMBA: Dimethylbenzanthracene; ROS: Reactive Oxygen Species; GPT: Glutamic Pyruvic Transaminase; ALT: Alanine amino Transferase; AST: Aspartate amino Transferase; PMN: Polymorphonuclear leucocytes; WBC: White Blood Cell; MIC: Minimum Inhibitory Concentration.



  • The plant constituents, pharmacological actions and ayurvedic aspects of Tinospora cordifolia is reviewed.

  • The plant contains active constituents like alkaloids, terpenoids etc.

  • The plant possesses antidiabetic, anticancer, antioxidant, antitoxin, hepatoprotective and immunomodulatory activities etc. These activities are correlated with the active constituents.

  • It’s use in ayurvedic system of medicine also reported.


Mr. Prashant Tiwari: He did B.Pharm from Guru Ghasidas Central University, Bilaspur, Chhattisgarh in 2008 and M.Pharm in Pharmacology from Siksha O Anusandhan deemed to be University, Bhubaneswar in 2010. Presently he is pursuing his PhD under Indian Council of Medical Research (ICMR) sponsored research project at Department of Pharmacology, School of Pharmaceutical Sciences, SOA deemed to be University, Bhubaneswar, Odisha.

Miss Puravi Nayak: She has completed her B.Pharm. (2017) at School of Pharmaceutical Sciences, Siksha O Anusandhan University, Bhubaneswar. Currently, she is pursuing her post-graduation in Pharmacology at SOA deemed to be University. She has participated and presented several research papers in IPC and few other national seminars.

Dr. Shakti Ketan Prusty: He is presently working as Assistant Professor at Department of Pharmacology, School of Pharmaceutical Sciences, Siksha O Anusandhan deemed to be University, Bhubaneswar, Odisha. He has published number of research and review articles in various journals indexed with SCI and Scopus. He has completed his PhD in pharmacy from SOA deemed to be University in the year 2017.

Dr. Pratap Kumar Sahu: Working as an Professor at School of Pharmaceutical Sciences, Siksha O Anusandhan Deemed to be University, Bhubaneswar, Odisha. He has completed his Post-graduation in Pharmacology (2001) from Jamia Hamdard, New Delhi, Ph.D. in Pharmaceutical Sciences (2007) from Utkal University and M.B.A. (HR) from IGNOU (2007). He has successfully completed one project funded by LSRB, DRDO and the work has been applied for patent. One ICMR sponsored project is ongoing under his supervision. He has published more than 70 research and review articles in reputed national and international journals. He is also a life member of various professional body like IPA, IPS, APTI, ISTE, ISP, IAPST, ISCA etc. His areas of research include Neuropharmacology, Neurodegenerative disorder, Neuroendocrinology etc.


The authors are grateful for providing financial aid in the form of fellowship by the Indian Council of Medical Research (ICMR), New Delhi, India (45/5/2013/BMS/TRM).


[1] Conflicts of interest CONFLICT OF INTEREST STATEMENT We declare that we have no conflict of interest.



Preeti S , author. Tinospora cordifolia (Amrita)-a miracle herb and lifeline too many diseases. Int J Med Aromat Plants. 2011;1(2):57–61


Pandey G , author. DraVyaguna Vijnana (Materia Medica-Vegetable Drugs) Part I.


Kokate CK, Purohit AP, Gokhale SB , authors. Pharmacognosy. 46th Edn. Nirali prakashan; New Delhi: 2010. p. 104–105


Raghunathan K , author. The aqueous extract of T. cordifolia caused reduction of blood sugar in alloxan induced hyperglycemic rats and rabbits. J Res Ind Med. 1969;3:203–11


Chadha YR , author. The wealth of India, raw materials. Vol. 10. Publication and Information Directorate; 1985


Kirtikar KR, Basu BD , authors. Indian Medicinal Plants. Vol.2. Lalit Mohan basu; Leader Road, Allahabad: 1933. p. 77


Khosa RL, Prasad S , authors. Pharmacognostical studies on Guduchi (Tinospora cordifolia Miers). J Res Ind Med. 1971;6:261–9


Sharma M, Kumar A , authors. Pharmacognostial Characterization of Some Selected Medicinal Plants of Semi-Arid Regions. J Pharmacogn Phytochemical. 2013;1(6)


Kirtikar KR, Basu BD , authors. Indian Medicinal Plants. 1975. p. 32nd Edn.


Nadkarni KM, Nadkarni AK , authors. Indian Meteria Medica. 3rd Edn. Popular Prakhasan; Bombay: 1976. 1. p. 45–52


Raghunathan K, Mitra RN , authors. Pharmacognosy of indigenous drugs, published by central council for research in Ayurveda and Siddha. New Delhi: 1982. 2. p. 687–95


Sharma PC, Yelne MB, Dennis TJ, Joshi A, Billore KV , authors. Database on medicinal plants used in Ayurveda. 2001;256


Charka CS , author; Shastri Rajeshwar Datta , editor. Part I & II. Hindi commentary by Pandey & Chaturvedi). Chaukhambha Vidyabhawan; Varanasi: 1961


Samhita SS , author. Commentary by Dalhana and Gayadasa. Varanasi: Chaukambha Orientalia; 1992


Vagbhata AH , author. With the commentaries, ‘Sarvangasundara’ of Arunadatta and ‘Ayurvedarasayana’ of Hemadri Annotated by Dr. Pt. Harishastri Paradakar Bhishgacharya. Reprint. 2002;21–2


Misra B, Nighantu BP , authors. Hindi commentary by KC Chunekar. Chowkhamba Vidya Bhavan; Varanasi: 1969. 1. p. 269


Aiyer KN, Kolammal M , authors. Pharmacognosy of Ayurvedic Drugs, Kerala. Department of Pharmacognosy; University of Kerala: 1963


Nadkarni KM, Nadkarni AK , authors. Indian Meteria Medica. 3rd Edn. Popular Prakhasan; Bombay: 1976. (1):p. 45–52


Pendse GP, Bhatt SK , authors. Chemical Examination of some Indian Medicinal Plants, Tinospora cordifolia, Solanum xanthocarpum and Fumaria officinalis in Bengal Pharmacopoea. Indian J Med Res. 1932;20(653)


Khosa RL, Prasad S , authors. Pharmacognostical studies on Guduchi (Tinospora cordifolia Miers). J Res Ind Med. 1971;6:261–9


Upadhyay AK, Kumar K, Kumar A, Mishra HS , authors. Tinospora cordifolia (Willd.) Hook. F. and Thoms (Guduchi)-validation of the Ayurvedic pharmacology through experimental and clinical studies. Int J Ayurveda Res. 2010;1(2):112


Rout GR , author. Identification of Tinospora cordifolia (Willd.) Miers ex Hook F. Thomas and Using RAPD Markers. Z Naturforsch C. 2006;61(1-2):118–22


Patel SS, Shah RS, Goyal RK , authors. Antihyperglycemic, antihyperlipidemic and anti-oxidant effects of Dihar, a polyherbal ayurvedic formulation in streptozotocin induced diabetic rats. Indian J Exp Biology. 2009;47:564–70


Gupta R, Sharma V , authors. Ameliorative effects of Tinospora cordifolia root extract on histopathological and biochemical changes induced by aflatoxin-B1 in mice kidney. Toxicol Int. 2011;18(2):94


Jagetia GC, Rao SK , authors. Evaluation of the antineoplastic activity of guduchi (Tinospora cordifolia) in Ehrlich ascites carcinoma bearing mice. Biol Pharm Bull. 2006;29(3):460–6


Patel MB, Mishra S , authors. Hypoglycemic activity of alkaloidal fraction of Tinospora cordifolia. Phytomedicine. 2011;18(12):1045–52


Sriramaneni RN, Omar AZ, Ibrahim SM, Amirin S, Zaini AM , authors. Vasorelaxant effect of diterpenoid lactones from Andrographis paniculata chloroform extract on rat aortic rings. Pharmacognosy Res. 2010;2(4):242–6


Yang S, Evens AM, Prachand S, Singh AT, Bhalla S, David K, et al. , authors. Mitochondrial-mediated apoptosis in lymphoma cells by the diterpenoid lactone andrographolide, the active component of Andrographis paniculata. Clin Cancer Res. 2010;16(19):4755–68


Zhao F, He EQ, Wang L, Liu K , authors. Anti-tumor activities of andrographolide, a diterpene from Andrographis paniculata, by inducing apoptosis and inhibiting VEGF level. J Asian Nat Prod Res. 2008;10(5):467–73


Kohno H, Maeda M, Tanino M, Tsukio Y, Ueda N, Wada K, et al. , authors. A bitter diterpenoid furanolactone columbin from Calumbae Radix inhibits azoxymethane-induced rat colon carcinogenesis. Cancer Lett. 2002;183(2):131–9


Dhanasekaran M, Baskar AA, Ignacimuthu S, Agastian P, Duraipandiyan V , authors. Chemopreventive potential of Epoxy clerodane diterpene from Tinospora cordifolia against diethyl nitrosamine-induced hepatocellular carcinoma. Invest New Drugs. 2009;27(4):347–55


Lv J, Xu D, Perkovic V, Ma X, Johnson DW, Woodward M, et al. , authors. TESTING Study Group. Corticosteroid therapy in IgA nephropathy. J Am Soc Nephrol. 2012;23(6):1108–16


McKeown E, Bykerk VP, De LF, Bonner A, Thorne C, Hitchon CA, et al. , authors. Quality assurance study of the use of preventative therapies in glucocorticoid-induced osteoporosis in early inflammatory arthritis: Results from the CATCH cohort. Rheumatology. 2012;51(9):1662–9


Sundarraj S, Thangam R, Sreevani V, Kaveri K, Gunasekaran P, Achiraman S, et al. , authors. γ-Sitosterol from Acacia nilotica L. induces G2/M cell cycle arrest and apoptosis through c-Myc suppression in MCF-7 and A549 cells. J Ethnopharmacol. 2012;141(3):803–9


Ly PT, Singh S, Shaw CA , authors. Novel environmental toxins: Steryl glycosides as a potential etiological factor for age‐related neurodegenerative diseases. J Neurosci. 2007;85(2):231–7


Karpova EA, Voznyi Y, Dudukina TV, Tsvetkova IV , authors. 4-Trifluoromethylumbelliferyl glycosides as new substrates for revealing diseases connected with hereditary deficiency of lysosome glycosidases. Biochem Int. 1991;24(6):1135–44


Kapil A, Sharma S , authors. Immunopotentiating compounds from Tinospora cordifolia. J Ethnopharmacol. 1997;58(2):89–95


Chen S, Wu K, Knox R , authors. Structure-function studies of DT-diaphorase (NQO1) and NRH: Quinone oxidoreductase (NQO2) 1. Free Radic Biol Med. 2000;29(3-4):276–84


Baldwin AS , author. Control of oncogenesis and cancer therapy resistance by the transcription factor NF-κB. J Clin Invest. 2001;107(3):241–6


Yang JH, Kondratyuk TP, Marler LE, Qiu X, Choi Y, Cao H, et al. , authors. Isolation and evaluation of kaempferol glycosides from the fern Neocheiropteris palmatopedata. Phytochem. 2010;71(5-6):641–7


Kim SK, Kim HJ, Choi SE, Park KH, Choi HK, Lee MW , authors. Anti-oxidative and inhibitory activities on nitric oxide (NO) and prostaglandin E 2 (COX-2) production of flavonoids from seeds of Prunus tomentosa Thunberg. Arch Pharm Res. 2008;31(4):424


Oliveira AM, Conserva LM, DeSouza FJN, Brito FD, Lemos RP, Barreto E , authors. Antinociceptive and anti-inflammatory effects of octacosanol from the leaves of Sabicea grisea var. grisea in mice. Int J Mol Sci. 2012;13(2):1598–611


Wang T, Liu YY, Wang X, Yang N, Zhu HB, Zuo PP , authors. Protective effects of octacosanol on 6-hydroxydopamine-induced Parkinsonism in rats via regulation of ProNGF and NGF signalling. Acta Pharmacol Sin. 2010;31(7):765–74


Thippeswamy G, Sheela ML, Salimath BP , authors. Octacosanol isolated from Tinospora cordifolia down regulates VEGF gene expression by inhibiting nuclear translocation of NF-< kappa> B and its DNA binding activity. Eur J Pharmacol. 2008;588(2-3):141–50


Maurya R, Handa SS , authors. Tinocordifolin, a sesquiterpene from Tinospora cordifolia1. Phytochem. 1998;49(5):1343–6


Ghosh AK, Chapsal BD, Weber IT, Mitsuya H , authors. Design of HIV protease inhibitors targeting protein backbone: an effective strategy for combating drug resistance. Acc Chem Res. 2007;41(1):78–86


Mukherjee R, De UK, Ram GC , authors. Evaluation of mammary gland immunity and therapeutic potential of Tinospora cordifolia against bovine subclinical mastitis. Trop Anim Health Prod. 2010;42(4):645–51


Devprakash SK, Subburaju T, Gurav S, Singh S , authors. Tinospora cordifolia: A review on its ethnobotany, phytochemical and pharmacological profile. Asian J Biomed Pharmaceut Sci. 2011;4(1):291–302


Jagetia GC, Nayak V, Vidyasagar MS , authors. Evaluation of the antineoplastic activity of guduchi (Tinospora cordifolia) in cultured HeLa cells. Cancer Lett. 1998;127(1):71–82


Singh RP, Banerjee S, Kumar PV, Raveesha KA, Rao AR , authors. Tinospora cordifolia induces enzymes of carcinogen/drug metabolism and antioxidant system and inhibits lipid peroxidation in mice. Phytochem. 2006;13(1-2):74–84


Thippeswamy G, Salimath BP , authors. Induction of caspase-3 activated DNase mediated apoptosis by hexane fraction of Tinospora cordifolia in EAT cells. Environ Toxicol Pharmacol. 2007;23(2):212–20


Chaudhary R, Jahan S, Goyal PK , authors. Chemopreventive potential of an Indian medicinal plant (Tinospora cordifolia) on skin carcinogenesis in mice. J Environ Pathol Toxicol Oncol. 2008;27(3):233–43


Rao SK, Rao PS, Rao BN , authors. Preliminary investigation of the radiosensitizing activity of guduchi (Tinospora cordifolia) in tumor‐bearing mice. Phytother Res. 2008;22(11):1482–9


Verma R, Chaudhary HS, Agrawal RC , authors. Evaluation of anticarcinogenic and antimutagenic effect of Tinospora cordifolia in experimental animals. J Chem Pharm Res. 2011;3(6):877–81


Ali H, Dixit S , authors. Extraction optimization of Tinospora cordifolia and assessment of the anticancer activity of its alkaloid palmatine. Scientific World Journal. 2013;28:376216


Mishra R, Kaur G , authors. Aqueous ethanolic extract of Tinospora cordifolia as a potential candidate for differentiation-based therapy of glioblas tomas. PLoS One. 2013;8(10):e78764


Bala M, Pratap K, Verma PK, Singh B, Padwad Y , authors. Validation of ethno medicinal potential of Tinospora cordifolia for anticancer and immunomodulatory activities and quantification of bioactive molecules by HPTLC. J Ethnopharmacol. 2015;175:131–7


Gupta R, Sharma V , authors. Ameliorative effects of Tinospora cordifolia root extract on histopathological and biochemical changes induced by aflatoxin-B1 in mice kidney. Toxicol Int. 2011;18(2):94


Sharma V, Pandey D , authors. Protective role of Tinospora cordifolia against lead-induced hepatotoxicity. Toxicol Int. 2010;17(1):12


Sharma V, Pandey D , authors. Beneficial effects of Tinospora cordifolia on blood profiles in male mice exposed to lead. Toxicol Int. 2010;17(1):8


Hamsa TP, Kuttan G , authors. Tinospora cordifolia ameliorates urotoxic effect of cyclophosphamide by modulating GSH and cytokine levels. Exp Toxicol Pathol. 2012;64(4):307–14


Shivananjappa MM , author. Abrogation of maternal and fetal oxidative stress in the streptozotocin-induced diabetic rat by dietary supplements of Tinospora cordifolia. Nutrition. 2012;28(5):581–7


Stanely P, Prince M, Menon VP , authors. Hypoglycaemic and other related actions of Tinospora cordifolia roots in alloxan-induced diabetic rats. J Ethnopharmacol. 2000;70(1):9–15


Umamaheswari S, Prince Mainzen PS , authors. Antihyperglycemic effect of ‘Ilogen-Excel’, an ayurvedic herbal formulation in streptozotocin-induced diabetes mellitus. Acta Pol Pharm. 2007;64:53–61


Stanely Mainzen Prince P, Menon VP , authors. Hypoglycaemic and hypolipidaemic action of alcohol extract of Tinospora cordifolia roots in chemical induced diabetes in rats. Phytother Res. 2003;17(4):410–3


Babu PS, Prince P , authors. Antihyperglycaemic and antioxidant effect of hyponidd, an ayurvedic herbomineral formulation in streptozotocin‐induced diabetic rats. J Pharm Pharmacol. 2004;56(11):1435–42


Patel SS, Shah RS, Goyal RK , authors. Antihyperglycemic, antihyperlipidemic and antioxidant effects of Dihar, a polyherbal ayurvedic formulation in streptozotocin induced diabetic rats. Indian J Exp Biol;2009;47(7):564–70


Sangeetha MK, Raghavendran HR, Gayathri V, Vasanthi HR , authors. Tinospora cordifolia attenuates oxidative stress and distorted carbohydrate metabolism in experimentally induced type 2 diabetes in rats. J Nat Med. 2011;65(3-4):544–50


Chougale AD, Ghadyale VA, Panaskar SN, Arvindekar AU , authors. Alpha glucosidase inhibition by stem extract of Tinospora cordifolia. Journal of Enzyme Inhibition and Med Chem. 2009;24(4):998–1001


Singh CS, Singh AK, Khandelwal S, Vishwkarma R , authors. Anti-Diabetic Activity of Ethanolic Extract of Tinospora cordifolia Leaves. Int J of Drug Discov and Herb Res. 2013;3(1):601–4


Sharma U, Bala M, Kumar N, Singh B, Munshi RK, Bhalerao S , authors. Immunomodulatory active compounds from Tinospora cordifolia. J Ethnopharmacol. 2012;141(3):918–26


Aher V, Wahi AK , authors. Biotechnological approach to evaluate the immunomodulatory activity of ethanolic extract of Tinospora cordifolia stem (mango plant climber). Iran J Pharm Res. 2012;11(3):863–72


More P, Pai K , authors. In vitro NADH-oxidase, NADPH-oxidase and myeloperoxidase activity of macrophages after Tinospora cordifolia (guduchi) treatment. Immunopharmacol Immunotoxicol. 2012;34(3):368–72


Upadhyaya R, Pandey RP, Sharma V, Verma Anita K , authors. Assessment of the multifaceted immunomodulatory potential of the aqueous extract of Tinospora cordifolia. Res J Chem Sci. 2011;71–9


Castillo AL, Ramos JD, De Francia JL, Quilala PF, Dujunco MU , authors. Immunomodulatory effects of Tinospora cordifolia lotion on interleukin-1, interleukin-6 and interleukin-8 levels in scabies-infected paediatric patients: a single blind, randomized trial. Int J Pharm Sci. Drug Res. 2014;6(3):204–10


Sudhakaran DS, Srirekha P, Devasree LD, Premsingh S, Michael RD , authors. Immunostimulatory effect of Tinospora cordifolia Miers leaf extract in Oreochromis mossambicus. Indian J Exp Biol. 2006;44:726–32


Jahfar M , author. Glycosyl composition of polysaccharide from Tinospora cordifolia. Acta pharmaceutica (Zagreb, Croatia). 2003;53(1):65–9


Raghu R, Sharma D, Ramakrishnan R, Khanam S, Chintalwar GJ, Sainis KB , authors. Molecular events in the activation of B cells and macrophages by a non-microbial TLR4 agonist, G1-4A from Tinospora cordifolia. Immunol Lett. 2009;123;1:60–71


Koppada R, Norozian FM, Torbati D, Kalomiris S, Ramachandran C, Totapally BR , authors. Physiological Effects of a Novel Immune Stimulator Drug,(1, 4)‐α‐d‐Glucan, in Rats. Basic Clin Pharmacol Toxicol. 2009;105(4):217–21


Salkar K, Suthar A, Chotalia C , authors. Study of Immunomodulatory activity of Tinospora cordifolia extract. Int J Pharm Bio Sci. 2014;3(4):880–3


Aher VD, Wahi A , authors. Pharmacological study of Tinospora cordifolia as an immunomodulator. Int J Curr Pharm Res. 2010;2(4):52–4


Umretia B, Vaishnav P, Patgiri B, Shukla V , authors. Immunomodulatory activity of Guduchi Ghana (Aqueous Extract of Tinospora cordifolia Miers). NJIRM. 2013;4(3):90–6


Singh K, Panghal M, Kadyan S, Chaudhary U, Yadav JP , authors. Antibacterial activity of synthesized silver nanoparticles from Tinospora cordifolia against multi drug resistant strains of Pseudomonas aeruginosa isolated from burn patients. J Nanomed Nanotechnol. 2014;5(2):1–6


Narayanan A, Raja S, Ponmurugan K, Kandekar S, Natarajaseenivasan K, Maripandi A, et al. , authors. Antibacterial activity of selected medicinal plants against multiple antibiotic resistant uropathogens: a study from Kolli Hills, Tamil Nadu, India. Benef Microbes. 2011;2(3):235–43


Jeyachandran R, Xavier TF, Anand SP , authors. Antibacterial activity of stem extracts of Tinospora cordifolia (Willd) Hook. F and Thomson. Ancient Sci Life. 2003;23(1):40–3


Tambekar DH, Khante BS, Chandak BR, Titare AS, Boralkar SS, Aghadte SN , authors. Screening of antibacterial potentials of some medicinal plants from Melghat forest in India. Afr J Tradit Complement Altern Med. 2009;6:228–32


Duraipandiyan V, Ignacimuthu S, Balakrishna K, AL-Harbi NA , authors. Antimicrobial activity of Tinospora cordifolia: an ethno medicinal plant. Asian J Tradit Med. 2012;7(2):59–65


Purandare H, Supe A , authors. Immunomodulatory role of Tinospora cordifolia as an adjuvant in surgical treatment of diabetic foot ulcers: A prospective randomized controlled study. Indian J Med Sci. 2007;61(6):347–55


Shanthi V, Nelson R , authors. Antibacterial activity of Tinospora cordifolia (Willd) Hook. F. Thoms on urinary tract pathogens. Int J Curr Microbial App Sci. 2013;2(6):190–4


Bonvicini F, Mandrone M, Antognoni F, Poli F, Angela Gentilomi G , authors. Ethanolic extracts of Tinospora cordifolia and Alstonia scholaris show antimicrobial activity towards clinical isolates of methicillin-resistant and carbapenemase-producing bacteria Nat Prod Res. 2014;28(18):1438–45


Narayanan A, Raja S, Ponmurugan K, Kandekar S, Natarajaseenivasan K, Maripandi A, et al. , authors. Antibacterial activity of selected medicinal plants against multiple antibiotic resistant uropathogens: a study from Kolli Hills, Tamil Nadu, India. Benef Microbes. 2011;2(3):235–43


Bhawya D, Anilakumar KR , authors. In Vitro Antioxidant Potency of Tinospora cordifolia (gulancha) in Sequential Extracts. Int J Pharm Biolo Arch. 2010;1(5):448–56


Sivakumar V, Rajan MD , authors. Antioxidant effect of Tinospora cordifolia extract in alloxan-induced diabetic rats. Indian J Pharm Sci. 2010;72(6):795–8


Stanley MPP, Menon VP , authors. Antioxidant action of Tinospora cordifolia root extract in alloxan diabetic rats. Phytother Res. 2001;15(3):213–8


Gacche RN, Dhole NA , authors. Profile of aldose reductase inhibition, anti-cataract and free radical scavenging activity of selected medicinal plants: an attempt to standardize the botanicals for amelioration of diabetes complications. Food Chem Toxicol. 2011;49(8):1806–13


Rawal A, Muddeshwar M, Biswas S , authors. Effect of Rubia cordifolia, Fagonia cretica linn, and Tinospora cordifolia on free radical generation and lipid peroxidation during oxygen-glucose deprivation in rat hippocampal slices. Biochem Biophys Res Commun. 2004;324(2):588–96


Upadhyay N, Ganie SA, Agnihotri RK, Sharma R , authors. Free radical scavenging activity of Tinospora cordifolia (Willd.). Miers. J Pharmacog Phytochem. 2014;3(2):63–9


Subramanian M, Chintalwar GJ, Chattopadhyay S , authors. Antioxidant properties of a Tinospora cordifolia polysaccharide against iron-mediated lipid damage and γ-ray induced protein damage. Redox Rep. 2002;7(3):137–43


Jayaprakash R, Ramesh V, Sridhar MP, Sasikala C , authors. Antioxidant activity of ethanolic extract of Tinospora cordifolia on N-nitrosodiethylamine (diethyl nitrosamine) induced liver cancer in male Wister albino rats. J Pharm Bioallied Sci. 2015;7(S1):S40–5


Kapur P, Wuttke W, Jarry H, Seidlova-Wuttke D , authors. Beneficial effects of β-Ecdysone on the joint, epiphyseal cartilage tissue and trabecular bone in ovariectomized rats. Phytomedicine. 2010;17(5):350–5


Patel A, Bigoniya P, Singh CS, Patel NS , authors. Radioprotective and cytoprotective activity of Tinospora cordifolia stem enriched extract containing cordifolioside-A. Indian J Pharmacol. 2013;45(3):237


Goel HC, Kumar Prem I, Rana SV , authors. Free radical scavenging and metal chelation by Tinospora cordifolia, a possible role in radioprotection. Indian J Exp Biol. 2002;40:727–34


Bafna PA, Balaraman R , authors. Anti-ulcer and anti-oxidant activity of pepticare, a herbomineral formulation. Phytomedicine. 2005;12(4):264–70


Gupta GD, Sujatha N, Dhanik A, Rai NP , authors. Clinical evaluation of Shilajatu Rasayana in patients with HIV infection. Ayu. 2010;31(1):28–32


Kalikar MV, Thawani VR, Varadpande UK, Sontakke SD, Singh RP, Khiyani RK , authors. Immunomodulatory effect of Tinospora cordifolia extract in human immunodeficiency virus positive patients. Indian J Pharmacol. 2008;40(3):107–10


Akhtar S , author. Use of Tinospora cordifolia in HIV infection. Indian J Pharmacol. 2010;42(1):57


Abiramasundari G, Sumalatha KR, Sreepriya M , authors. Effects of Tinospora cordifolia (Menispermaceae) on the proliferation, osteogenic differentiation and mineralization of osteoblast model systems in vitro. J Ethnopharmacol. 2012;141(1):474–80


Gao L, Cai G, Shi X , authors. β-Ecdysterone induces osteogenic differentiation in mouse mesenchymal stem cells and relieves osteoporosis. Biol Pharm Bull. 2008;31(12):2245–9


Kaur M, Singh A, Kumar B , authors. Comparative antidiarrheal and antiulcer effect of the aqueous and ethanolic stem bark extracts of Tinospora cordifolia in rats. J Adv Pharm Technol Res. 2014;5(3):122–8


Goel B, Pathak N, Nim DK, Singh SK, Dixit RK, Chaurasia R , authors. Clinical evaluation of analgesic activity of guduchi (Tinospora cordifolia) using animal model. J Clin Diagn Res. JCDR. 2014;8(8):HC01–4


Wani JA, Achur RN, Nema RK , authors. Phytochemical screening and aphrodisiac activity of Asparagus racemosus. Studies. 2011;8(9):21–26


Sengupta M, Sharma GD, Chakraborty B , authors. Effect of aqueous extract of Tinospora cordifolia on functions of peritoneal macrophages isolated from CCl 4 intoxicated male albino mice. BMC Complement Altern Med. 2011;11(1):102


Kumara V, Mahdi F, Chander R, Husain I, Khanna AK, Singh R, et al. , authors. Tinospora cordifolia regulates lipid metabolism in allaxon induced diabetes in rats. Int J Pharm Life Sci. 2013;4(10)


Kosaraju J, Chinni S, Roy PD, Kannan E, Antony AS, Kumar MS , authors. Neuroprotective effect of Tinospora cordifolia ethanol extract on 6-hydroxy dopamine induced Parkinsonism. Indian J Pharmacol. 2014;46(2):176–80


Patgiri B, Umretia BL, Vaishnav PU, Prajapati PK, Shukla VJ, Ravishankar B , authors. Anti-inflammatory activity of Guduchi Ghana (aqueous extract of Tinospora cordifolia Miers.). Ayu. 2014;35(1):108–10


Antonisamy P, Dhanasekaran M, Ignacimuthu S, Duraipandiyan V, Balthazar JD, Agastian P, et al. , authors. Gastroprotective effect of epoxy clerodane diterpene isolated from Tinospora cordifolia Miers (Guduchi) on indomethacin-induced gastric ulcer in rats. Phytomedicine. 2014;21(7):966–9


Jayaprakash R, Ramesh V, Sridhar MP, Sasikala C , authors. Antioxidant activity of ethanolic extract of Tinospora cordifolia on N-nitrosodiethylamine (diethyl nitrosamine) induced liver cancer in male Wister albino rats. J Pharm Bioallied Sci. 2015;7(S1):S40


Patel A, Bigoniya P, Singh CS, Patel NS , authors. Radioprotective and cytoprotective activity of Tinospora cordifolia stem enriched extract containing cordifolioside-A. Indian J Pharmacol. 2013;45(3):237–43


Sivasubramanian A, Gadepalli NKK, Rathnasamy R, Campos AM , authors. A new antifeedant clerodane diterpenoid from Tinospora cordifolia. Nat Prod Res. 2013;27(16):1431–6


Stanca MH, Nagy A, Tosa M, Vlad L , authors. Hepatoprotective effects of orally administered melatonin and Tinospora cordifolia in experimental jaundice. Chirurgia (Bucharest, Romania: 1990). 2011;106(2):205–10. DOI: DOI10.21614/chirurgia.112.6.664


Gupta A, Singh Karchuli M, Upmanyu N , authors. Comparative evaluation of ethanolic extracts of Bacopa monnieri, Evolvulus alsinoides, Tinospora cordifolia and their combinations on cognitive functions in rats. Curr Aging Sci. 2013;6(3):239–43


Jain VK, Shete A , authors. Antipsychotic activity of aqueous ethanolic extract of Tinospora cordifolia in amphetamine challenged mice model. J Adv Pharm Technol Res. 2010;1(1):30–3


Dhingra D, Goyal PK , authors. Evidences for the involvement of monoaminergic and GABAergic systems in antidepressant-like activity of Tinospora cordifolia in mice. J Pharm Pharm Sci. 2008;70(6):761–7


Kapur P, Jarry H, Wuttke W, Pereira BM, Seidlova-Wuttke D , authors. Evaluation of the antiosteoporotic potential of Tinospora cordifolia in female rats. Maturitas. 2008;59(4):329–38


Gupta RS, Sharma A , authors. Antifertility effect of Tinospora cordifolia stem extract in male rats. Indian J Exp Biol. 2003;41(8):885–9


Tiwari M, Dwivedi UN, Kakkar P , authors. Tinospora cordifolia extract modulates COX-2, iNOS, ICAM-1, pro-inflammatory cytokines and redox status in murine model of asthma. J Ethnopharmacol. 2014;153(2):326–37


Mishra R, Kaur G , authors. Aqueous ethanolic extract of Tinospora cordifolia as a potential candidate for differentiation-based therapy of glioblastomas. PLoS One. 2013;8(10):e78764


Badar VA, Thawani VR, Wakode PT, Shrivastava MP, Gharpure KJ, Hingorani LL, et al. , authors. Efficacy of Tinospora cordifolia in allergic rhinitis. J Ethnopharmacol. 2005;96(3):445–9


Nadig PD, Revankar RR, Dethe SM, Narayanswamy SB, Aliyar MA , authors. Effect of Tinospora cordifolia on experimental diabetic neuropathy. Indian J Pharmacol. 2012;44(5):580–3


Dhanasekaran M, Baskar AA, Ignacimuthu S, Agastian P, Duraipandiyan V , authors. Chemopreventive potential of Epoxy clerodane diterpene from Tinospora cordifolia against diethyl nitrosamine-induced hepatocellular carcinoma. Invest New Drugs. 2009;27(4):347–55


Mishra R, Kaur G , authors. Tinospora cordifolia induces differentiation and senescence pathways in neuroblastoma cells. Mol Neurobiol. 2015;52(1):719–33


Jeyachandran R, Xavier TF, Anand SP , authors. Antibacterial activity of stem extracts of Tinospora cordifolia (Willd) Hook. F and Thomson. Anc Sci Life. 2003;23(1):40–3