Acne is caused by the effects of hormones on the pilosebaceous unit, consisting of a hair follicle and sebaceous gland. The normal skin follicles were blocked and an overgrowth by bacteria, Propionibacterium acnes causes destruction of the lining of the follicle causes follicular material enter into the dermis, causing an inflammatory response.1,2 It is a multi factorial disease affecting the pilosebaceous follicles and arises from an enhanced sebum excretion.3 Mainly topical and systemic therapies are involved in the treatment of Acne vulgaris, which act either by inhibiting bacteria (Propionibacterium acnes) involved in acne. Systemic therapies include oral antibiotic like doxycycline, erythromycin, immediate or by extended-release minocycline and tetracycline. Side effects of systemic medication are stomach, dizziness and teratogenesity. Topical therapies include retinoid like tretinoin and isotretinoin which causes skin irritation, erythema and peeling. Extensive use of topical and oral clindamycin has resulted in increasing clindamycin resistant microorganism which has increased inappropriate therapeutic responses.4
Therefore, dapsone has been introduced in the management of acne. Dapsone has longer half-life, with least side effects and interactions with other drugs and safety in pregnancy.5 Dapsone (4-[4-aminobenzene) sulfonyl] aniline) is an antimicrobial agent with bacteriostatic action. It inhibits the synthesis of dihydrofolic acid by competing with para-aminobenzoic acid for the active site of dihydropteorate synthetase, thus resembling the action of sulphonamide.6 The mechanism of topical dapsone in treatment of acne vulgaris may result from a combination of both anti-inflammatory and anti-microbial effects.6,7 Dapsone is a BCS class II drug with low aqueous solubility.8 Oral administration of dapsone has several side effects, including hemolyticanemia, nausea and headache. Adverse effects of dapsone are related to the production of metabolites. In the liver dapsone is acetylated by N-acetyltransferase which produces monoacetyldapsone, and upon enzymatic hydroxylation, dapsone hydroxylamine is produced, that is primarily responsible for the development of adverse effects.9 These side effects diminish its practicability to use it by the oral route for treating skin diseases. Because of unsuitability of dapsone for the oral route; topical route is another alternative. Dapsone is marketed as gel; the bioavailbitliy of dapsone can be improved by incorporating drug in microemulsion based gel. Microemulsion as colloidal nanosize carrier system is a promising vehicle for topical administration of drug with poor water solubility. Microemulsion had demonstrated improved transdermal permeation, thermodynamically stable over conventional topical preparations such as gel, cream and ointment. Microemulsion acts as penetration enhancer by interacting with stratum corneum, changing structural organization of lipid layer and consequently increasing transdermal drug permeation.10,11 The viscosity played an important role in promoting the drug retention on the stratum corneum and viable epidermis. However, microemulsion had very low viscosity and cannot retain at the affected area. Retention time of microemulsion can be increased by adding gelling agent. Some of the gelling agent such as carbapol, HPMC K-15, poloxamer-407 have been selected to increase the appropriate viscosity of microemulsion to enhance skin permeation, retention and form microemulsion based gel to improve topical applicability. In the present study attempt was made to formulate microemulsion based gel of dapsone using oil- 6%, Smix- 32%, Water-62% and poloxamer 407-18% w/w as gelling agent. Prepared gel was characterized for pH, viscosity, percent drug content, spreadability and in- vitro diffusion study.
MATERIALS AND METHODS
Dapsone was purchased from market. Capryol 90 provided as gift sample by Gattefosse India Pvt. Ltd., Mumbai, India. Kolliphor EL and Polaxomer 407 were provided as gift sample by BASF, Mumbai, India. N- methyl-2 pyrrolidone, PEG 400, Carbopol 934, Carbapol 940 and HPMC K100M were purchased from Research Laboratory, India. All other chemicals and reagents were of analytical grade.
Solubility of Dapsone
The saturation solubility of dapsone was determined by shaking method. Excess drug was added in 5 ml vial containing 2 ml distilled water and methanol. After proper mixing of the mixture the vial was kept in an orbital shaker at 37 ± 1°C for 24 h. The resulting solution was filtered through Whatman filter paper, diluted and absorbance was recorded at 296 nm.12
Formulation of Microemulsion of Dapsone Solubility study
The solubility of Dapsone was determined in various oils, surfactants, and co-surfactant. An excess amount of Dapsone was added in 2 mL of the selected oils, surfactants and cosurfactants taken in 5 mL stoppered vial separately, and mixed by vortexing. The vials were then kept at 37 ± 1.0°C in an orbital shaker for 48h to achieve equilibrium. The equilibrated samples were removed from shaker and centrifuged at 402×g for 15 min. The supernant was filtered through Whatman filter paper. The absorbance of these filtrates was noted using UV spectrometer and the concentration of dapsone was calculated.13
Construction of Pseudo-ternary phase diagram
Pseudo-ternary phase diagrams were constructed by titrating mixtures of oil and Smix (mixture of surfactant and co-surfactant) with water at room temperature. For this, the selected surfactant and co-surfactant were blended together in different ratios like 1:1, 1:2 and 2:1. Smix was mixed with oil phase to give weight ratio of 9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7, 2:8 and 1:9 (w/w) by using magnetic stirrer. These mixtures were titrated slowly with distilled water taking care for proper stirring of liquid phases to achieve equilibrium. After being equilibrated, the mixtures were assessed visually for transparency and further titrated over the entire phase region. The pseudoternary phase diagram was constructed for each system by plotting concentration of oil, surfactant and cosurfactant, a proper region was selected for formulation of microemulsion system from constructed pseudoternary phase diagrams. Various pseudoternary phase diagrams consisting of different oil, surfactant and cosurfacatnt mixture, in different ratio were constructed by using Triplot software version 184.108.40.206,15
Selection of formulation from Pseudo-Ternary Phase diagram
Pseudo ternary phase diagrams constructed using Capryol 90 and N- methyl- 2 pyrrolidone as oil phase, Kolliphor EL as surfactant and Polyethylene Glycol 400 as co-solvent. Various compositions of oil, Smix and water were selected for microemulsion formulation based on following criteria:
Considering the solubility of the drug in the selected oil (mg/mL), the concentration of oil had to be sufficient to solubilize the drug equivalent to dose (1 gm/100 gm of microemulsion)
For formation of homogenous, clear, transparent microemulsion for selected quantity of oil, the optimum quantity of water and Smix had to be used for formulation of microemulsion.15
Preparation of Microemulsion
After the identification of microemulsion region in the phase diagram, the microemulsion formulations were selected at desired component ratios Table 1. Dapsone loaded microemulsion was prepared by dissolving dapsone in N-methyl-2 pyrrolidone + Capryol 90 (1:1) with stirring. The required quantity of surfactant and cosurfacant (Kolliphor EL + PEG 400) were added and the mixture was stirred to yield a homogenous solution. To this solution, water was added to yield a microemulsion.15,16
Preparation of microemulsion based gel
The poloxamer-407 as a gel matrix was used to prepare the microemulsion- based gel for improving the viscosity of microemulsion for topical delivery of dapsone. The poloxamer-407 (18% w/w) dispersed in water and then kept in freezer for 24 h. Then the poloxamer- 407 solution was mixed with microemulsion under stirring. As the microemulsion was added to the solution of poloxamer- 407, the viscosity of microemulsion based gel was increased. The gel was then subjected to physicochemical evaluation, diffusion study and stability studies.16-19
Evaluation of microemulsion and microemulsion based gel
Microemulsions were evaluated for percent transmittance, conductance, globule size and zeta potential measurement. Microemulsion and microemulsion based gel both were evaluated for pH, viscosity, percent drug content and spreadability study.20,21
Optimized formulation was stored at ambient environmental conditions 40 ± 2°C and 75 ± 5% RH for 30 days. The samples were withdrawn on 0, 15 and 30 days of storage condition and evaluated for pH, drug content and in-vitro drug permeation study.20
|Formulation code||Composition (%w/w) of different component in micromeulsion with Smix|
|% Oil*||% Smix||% Water|
In- vitro diffusion study of microemulsion and microemulsion based gel
The study was performed using Franz diffusion cells with dialysis membrane. The recipient compartment had total capacity of 35 mL. Microemulsion and microemulsion based gel equivalent to 10 mg of dapsone was placed on the donor compartment and the receptor compartment was filled with mixture of phosphate buffer solution (pH 7.4) and 30% methanol, maintained at 37 ± 1°C. For in-vitro diffusion studies, artificial dialysis membrane was soaked in the same buffer solution for 24 h before mounting on the diffusion cells. Receptor liquid was withdrawn after each hour and sink condition was maintained by replacing liquid kept at same temperature. Dapsone concentration was assayed by UV spectrophotometer.22
Ex-vivo skin permeation study
Male albino Wistar rats skin was used for permeation study. Skin permeation study was carried out as follows:
Abdominal skins were obtained from male rat after sacrificing by chloroform. The hair on the skin was removed. The skin was carefully excised and the subcutaneous tissue was removed surgically and the dermis side was wiped with isopropyl alcohol to remove adhering fat. Then the skin was washed with water, wrapped in aluminium foil and stored in freezer for further use. In-vitro skin permeation study was performed by using Franz diffusion cells with diffusion area of 4.9 cm2. The excised skin samples (dorsal side) of rat were placed between the donor and the receptor compartment of Franz diffusion cells with the stratum corneum facing the donor compartment. Then, 1 g of microemulsion based gel or simple dapsone gel containing 1% (w/w) dapsone was applied on the donor compartment. The receptor compartment was filled with phosphate buffer (pH 7.4) and 30% methanol maintained at 37±1°C with stirring at 100 rpm. At predetermined time intervals (1 h), 1mL receptor medium was withdrawn up to 8 h. All samples were analyzed by UV spectrophotometer.23
Permeation data analysis
The cumulative amount of drug permeated per unit skin surface area plotted against time and slope of the linear portion of the plot was estimated as the steady state flux (Jss). Permeability coefficient was calculated as
Where, Cv is the total donar concentration of the solute.
Skin irritation test
Skin irritation study of Dapsone loaded microemulsion based gel was carried out by using Draize patch test on Albino Wistar rats of 180-250 g weight. Rats were selected in two groups of five animals each under laboratory condition of temperature and light/dark cycle. Back side of rat was shaved and optimized gel was applied on area of 2×2 cm to study skin irritation study for three days. Approximately 24, 48 and 72h after application, animals were examined for signs of irritation.26,27
Saturation solubility of dapsone in solvent, oil, surfactant and cosurfactant:
Being a BCS class II drug dapsone has very low solubility in water. The saturation solubility of Dapsone in distilled water was found to be 0.24 ± 0.056 mg/mL and in methanol was 49.76 ± 0.68 mg/mL.
Because of poor water solubility, solubility of dapsone in the oily phase of microemulsion is very critical because it may affect the stability as well as the percutaneous delivery performance of the formulation. To screen appropriate solvents for the preparation of microemulsions, the solubility of dapsone in various oils, non-ionic surfactants and co-surfactant was measured Figure 1. The solubility of dapsone in N-methyl-2 pyrrrolidone was 120 mg/mL but solubility of dapsone in blend of Capryol 90 and N-methyl-2 pyrrrolidone was 250 mg/mL. Use of N-methyl-2 pyrrolidone provided a greater microemulsion region and higher solubilization of Dapsone. So, to improve the solubility of dapsone the blend of N- methyl- 2 pyrrolidone + Capryol 90 (1:1) was used. Amongst the various surfactants and cosurfactants screened, dapsone exhibited good solubility in nonionic surfactants such as Tween 80. Though dapsone was showing less solubility in Kolliphor EL than Tween 80, it was decided to select kolliphor EL further study as it was giving better transparent region in pseudoternary plot.
Construction of pseudoternary phase diagram
The phase diagrams of Capryol 90+ N- methyl- 2 pyrrolidone+Kolliphor EL+ PEG 400+water was shown in Figure 2. The transparent o/w microemulsion area was presented as a shaded region in the phase diagrams. The Smix ratio 1:1 had larger microemulsification region as compared to 1:2 and 2:1. The emulsified area was the lowest at ratio of 2:1 as the concentration of co-surfactant was less. From the microemulsion regions in the phase diagrams, the microemulsion formulations were selected at different component ratios. The microemulsions containing excess amount of drug were prepared and evaluated. The effect of amount of surfactant: cosurfactant and oil phase on globule size of drug‑loaded microemulsions were investigated.
Evaluation parameter for microemulsion
Microemulsion had pH values from 5.8 to 6. pH of all these formulations were in the range of pH of skin (4 to 7).
The conductance of microemulsion formulations was measured using conductometer. It was observed that as the percentage of water was increased in microemulsion, the electrical conductivity also increased. The conductance of microemulsions was found in Table 2 the range of 14-16 (microsiemens/cm). Estimation of dapsone content in microemulsion formulation was carried out by the UV spectrometric method. The content of dapsone in various microemulsion was found to be in range of 80- 100%. Percent transmittance in microemulsion formulation was carried out by UV spectrometric method at 630 nm. The percentage transmittance of dapsone microemulsion formulations was found to be in the range of 97-100%.
Droplet size of microemulsion formulation is critical parameter. The smaller the droplet size, the larger will be interfacial surface area provided for drug permeation.21,27 Globule size of (F2) formulation was found to be 27.53 nm Table 2. The viscosity of optimized microemulsion (F2) was found to be 120 cps at 100 rpm which was determined by using Brookfield viscometer (DV II + PRO) with small sample volume adaptor. Zeta potential of optimized microemulsion (F2) was found was found to be-14.6 mV.
In-vitro diffusion of microemulsion
Figure 3 represent the cumulative percent drug permeated from the formulation F1 to F6 in 6 h through dialysis membrane shows that drug permeation greatly depends on the composition of microemulsion. After 6 h of permeation study through dialysis membrane, amount of drug permeated (72.20%) was maximum for F2 formulation.
|Formulation||pH||Spreadability gm cm/sec||Percent drugcontent*|
Evaluation parameter for microemulsion based gel
The pH of all the microemulsion based gel formulation was found to be in the range of 5.8-6.2. These pH values were considered to be acceptable since the skin pH ranges between 4-7. Although good spreadability was observed for all the formulations, the formulation gelled with polaxomer - 407 showed better spreadability in range of 14- 30 gm/cm/sec. Estimation of drug content in microemulsion based gel formulation was carried out by UV spectrophotometer. The content of dapsone in various microemulsion based gel was found to be in range of 80- 97% Table 3. Drug content study revealed that no degradation of drug was observed when formulated as microemulsion based gel.
Viscosity of all gels was determined by Brook filed viscometer by using spindle S- 94. Viscosity of all gel at 20 rpm was found to be FG1-32890, FG2-48500, FG3-78900, FG4-30300, FG5-33000 and FG6-65200 cps.
|Time interval (days)||pH||Drug content*||In vitro diffusion study*|
In-vitro diffusion of microemulsion based gel
The results of in-vitro diffusion study of microemulsion based gel through dialysis membrane using Franz diffusion cell are shown in Figure 4.
Among all the microemulsion based gel formulations, formulation FG2 (Oil: 6%, Smix: 32%, Water: 62%) showed good permeation rate through dialysis membrane (70 ± 0.09%), spread easily and it was found to be stable formulation and hence was selected as optimized formulation.
The result of stability study Table 4 revealed that there was no significant change in the microemulsion based gel formulation, no phase separation and no sign of precipitation of drug. Stability studies indicated that the preparation was stable at room temperature over the period of one month.
Ex-vivo skin permeation study
Ex-vivo skin permeation study showed higher drug release for microemulsion based gel (81.51 ± 0.42 %) as compare to simple gel of dapsone (58.98 ± 0.36%) at the end of 8 h Figure 5. This might be due to decreased globular size of microemulsion droplets (F2- 27.53 nm).
Kinetics of drug release from microemulsion and microemulsion based gel
The cumulative amount of drug released from selected dapsone microemulsion and microemulsion based gel formulation at different time interval was fitted to different models to find out the mechanism of drug release.
The correlation coefficients Table 5 showed that the kinetic of drug release from microemulsion and microemulsion based gel followed zero order model of kinetics.
The permeability parameters of different formulations are given in Table 6. Flux is the amount of drug permeated per unit area in unit time. The results showed that the optimized microemulsion formulation when gelled with poloxamer-407, the flux (Jss) and permeability coefficient (Kp) was greatly increased as compared to simple dapsone gel. At the end of 8 h microemulsion based gel showed flux of 392.43 ± 0.814 µg/cm2/h and cumulative drug release was found to be 81.51 ± 0.42%. Increase in flux as compare to simple dapsone gel with highest enhancement ratio (Er) 1.430, which indicates faster drug release from microemulsion based gel.
|Code||Zero Order||First order||Matrix||Peppas||Hix – Crow|
Skin irritation study of microemulsion based gel on rat
Frequent use of some topical formulation can induce irritation and damage the skin. Therefore, skin irritation studies are developed for topical formulation.
Treatment groups not showed any signs of skin irritation as reddening and inflammation at the site of application. Skin of rat was found to be free of any signs of irritation Figure 6. Thus it can be concluded that all selected microemulsion based gel of dapsone are safe for topical application.
Capryol 90 + N-methyl-2 pyrrolidone as oil phase (1:1) and Kolliphor EL + PEG 400 as the Smix for microemulsion showed better transparent region, because dapsone has good solubility in these solvents, which helped to get the transparent region in pseudo-ternary phase diagram. It was evident from the results that the droplet size is inversely proportional to the concentration of Smix and directly proportional to concentration of oil. A decrease in particle size with increase in Smix may be the result of more non-ionic surfactants (Smix) being available to solubilize the oil/water interface.
|Formulations||Flux (Jss±SD) (µg cm-2 h-1)||Permeability coefficient (Kp) cm h-1||Enhancement ratio (Er)|
|Dapsone microemulsion based gel||392.43 ± 0.814||0.03922±0.0065||1.430|
|Dapsone gel||274.4 ± 0.78||0.02474±0.0078|
Sticking of particles to each other (aggregation, coagulation and flocculation) or to the surrounding surface causes microemulsion instability. Sticking can be counteracted by strong electrostatic repulsion and the strength of that repulsion can be measured by the zeta potential. Negative zeta potential showed that globules of microemulsion had no charge, the system was stable. It was observed from the result of viscosity measurements that viscosity values of all microemulsion formulation were increased slightly with increase in concentration of Smix. Conductivity studies showed that addition of the appropriate amount of water phase into the formulation had no negative effects on system stability. In an unstable microemulsion system the conductivity values were reduced because of phase separation. Good spreadability is because of the loose gel matrix nature of microemulsion based gel. Increase in shear causes alignment of random molecules of gelling material thus decreasing internal resistance of material. The pH range for skin is 6-8. Measured pH values of all formulations were found to be compatible for topical formulations.
Higher percent drug permeation of F2 formulation was might be due to change in concentration of oil, Smix and water. Smaller particle size of F2 formulation permeated better drug as compared to other formulations. It has been shown that release rate from microemulsion based gel was higher as compared to simple dapsone gel. Greater permeability of drug from microemulsion based gel may be attributed to different factors, such as a) microemulsion with reduced particle size provides more surface area to release drug, thereby increase the rate of drug release, and b) various components of microemulsion also have penetration enhancing effect.
Enhance steady state flux of Microemulsion gel over the simple gel could be due to the permeation enhancer property of surfactant and cosurfactant present in microemulsion. Permeation enhancer causes reduction in diffusional barrier and disturbance in the stratum corneum structure. The microemulsion may simultaneously alter both the lipid and the polar pathways as a combined effect of both the lipophilic and hydrophilic domains of microemulsions. The lipophilic domain of the microemulsion can interact with the stratum corneum and facilitate permeation. The hydrophillic domain causes the hydration of the skin, which plays an important role in the percutaneous uptake of poorly soluble drug. When the aqueous fluid of the microemulsion enters the polar pathways, it increases the interlamellar volume of stratum corneum lipid bilayers, resulting in disruption of the interfacial structure. Since some lipid chains are covalently attached to corneocytes, hydration of these proteins will also lead to disorder of lipid bilayers. Similarly, swelling of the intercellular proteins may also disturb the lipid bilayers; a lipophilic drug like dapsone can then permeate more easily through the lipid pathway of the stratum corneum.28
The study revealed that microemulsion based gel prepared with Poloxamer-407 (18%) as a gelling agent imparts viscosity to the preparation to sustain the action of the drug by increasing the residence time. The contents of the microemulsion based gel was composed of Dapsone (1% w/w), N-methyl-2 pyrrolidone- Capryol 90 (1:1), (6% w/w), Kolliphor EL (32% w/w), poloxamer-407 (18% w/w) and water (62% w/w). The increased permeability of dapsone microemulsion based gel was observed 81.51 ± 0.42 % within 8 h compared to simple gel 58.98 ± 0.36%%. There was no change in drug content, in-vitro diffusion study of the microemulsion based gel after 30 days of stability testing. Conclusively, microemulsion based gel could be one of the approach to enhanced the solubility and to improve permeability of dapsone.