Aztreonam1,2 is a synthetic monobactam bactericidal antibiotic originally isolated from chromo bacterium violaceum. It is a white crystalline powder. Aztreonam is chemically (z)-2- [ (2- amino - 4-thiazolyl) [ [ (2S - 3S) - 2- methyl- 4- oxo -1- sulfo -3-azetidinyl ] carbomoyl ] methylene ] amino] oxy ] -2- methyl propionic acid, which is used in the treatment of life threatening infections with susceptible gram-negative aerobic organisms, especially Pseudomonas aeruginosa. Aztreonam acts by inhibiting bacterial cell wall peptidoglycan synthesis. Literature reviews3-11 revealed, no stability indicating method of estimation for aztreonam by high performance liquid chromatography12-14 has been reported so far except in biological fluids.
MATERIALS AND METHOD
Reagents and chemicals
Aztreonam was obtained from APL research lab Hyderabad. Methanol, water (LC-grade) and orthophosphoric acid were obtained from Merck. LC grade Acetonitrile were purchased from Molychem. Analytical grade sodium hydroxide (NaOH), Hydrochloric acid (HCl), were obtained from Fischer scientific. Hydrogen peroxide (H2O2) and 0.22 μm membrane filter were obtained from Sigma–Aldrich. Formulation was procured from the local market having strength of 500 mg/vial. All chemicals were of analytical or LC-grade. All the measurements were made using Waters HPLC. All the solutions were freshly prepared by using HPLC grade solvents.
Optimized chromatographic conditions
Instrument used: Waters HPLC with auto sampler and 2487 UV detector with Empower 2 software.
Stock and working solution
10 mg of aztreonam was taken in a 100 ml standard flask and the volume was made up to 100 ml with water to get a concentration of 100 μg/ml of aztreonam. From the above solution, further dilutions were made to get concentrations from 5-25 μg/ml.
Preparation of sample solution
The whole content of the vial was transferred to a 100 ml standard flask and dissolved in 10 ml of water for injection. Then the volume was made up to 100 ml using water (HPLC grade). From this 1 ml was taken and diluted to 10ml. From the above solution 2 ml was taken and diluted to 10 ml (100 μg/ml). The above solution was further diluted to get concentrations ranging from 5-25 μg/ml. The amount of drug present in the vial was calculated as follows.
The International Conference on Harmonization (ICH) guideline entitled stability testing of new drug substances and products requires that stress testing should be carried out to elucidate the inherent stability characteristics of the active substance. The aim of this work was to perform the stress degradation studies on aztreonam using the proposed method.
Preparation of stock
Accurately weighed and transferred 10 mg of aztreonam working standard into a 10 ml clean dry volumetric flask, added about 7 mL of Diluent and sonicated to dissolve it completely and made volume up to the mark with the same solvent.
Hydrolytic degradation under acidic condition
Pipetted out 0.75 ml of above solution into a 10ml volumetric flask and 3 ml of 0.1N HCl was added. Then, the volumetric flask was kept at 60°C for 6 hours and then neutralized with 0.1 N NaOH and made up to 10ml with diluent. Filtered the solution with 0.22 microns syringe filters and placed in vials.
Hydrolytic degradation under alkaline condition
Pipetted out 0.75 ml of above solution into a 10ml volumetric flask and 3ml of 0.1N NaOH was added in 10 ml of volumetric flask. Then, the volumetric flask was kept at 60°C for 6 h. Then neutralized with 0.1 N HCl and made up to 10ml with diluent. Filtered the solution with 0.22 microns syringe filters and placed in vials.
Thermal induced degradation
Aztreonam sample was taken in petridish and kept in Hot air oven at 110°C for 24 hrs. Then the sample was taken and diluted with diluents and injected into HPLC and analyzed.
Pipetted out 0.75 ml of above stock solution into a 10 ml volumetric flask and 1 ml of 3% w/v of hydrogen peroxide was added and the volume was made up to the mark with diluent. The volumetric flask was then kept at room temperature for 15 min. Filtered the solution with 0.45 microns syringe filters and placed in vials.
To study the reliability and accuracy of the method, recovery experiments were carried out. To the formulation equivalent to 10 μg/ml, standard aztreonam was added at the level of 50 % and 100 %. These were further diluted by the procedure followed in the estimation of formulation. The concentration of the drug present in the resulting sample solution was determined. The recovery procedure was repeated 3 times and the percentage recovery was calculated using the formula,
Method precision: A sample solution of aztreonam was prepared. A sample containing 15 μg/ml of Aztreonam was injected 3 times.
Intermediate/ruggdness: It was carried out by injecting a concentration (15 μg/ml) of the sample solution with different ‘make’ column and standard deviation was calculated.
Linearity and Range
Calibration curve was well established in the range of 5-25 μg/ml with correlation co-efficient value 0.9998
Limit of detection (LOD) and Limit of quantification (LOQ)
The LOD and LOQ of the developed method were determined by injecting progressively low concentration of the standard solution under optimized chromatographic conditions.
The LOD and LOQ of Aztreonam were found to be 20 ng/ml and 5 μg/ml respectively.
In the present study, mobile phase, standard and sample solutions were subjected to 3 days stability studies. The stabilities of these solutions were studied by performing the experiment, looking for change in retention time, resolution, tailing of the peaks etc when compared to the pattern of the chromatogram of freshly prepared solution. The solution stored under room temperature was stable for 2 hrs and under refrigeration for 24 hrs.
System suitability studies
The system suitability studies were carried out as specified in USP. These parameters include column efficiency, resolution, peak asymmetry factor, capacity factor, peak tailing factor and percentage co-efficient of variation for peak area or height of repetitive injection. Although USP requires only two of these criteria for method validation, parameters like column efficiency (N), resolution (Rs), capacity factor (K), selectivity factor (ɑ) and peak asymmetry factor (As) were calculated in this study.
RESULTS AND DISCUSSION
Different ionic strengths such as 10, 25 mM solutions of potassium dihydrogen phosphate, adjusted to pH 2.6 were initially employed for the separation of aztreonam in the ratio of 20:80 (methanol: buffer). In all the cases aztreonam showed split peak with tailing, but in the case of buffer pH 3: Acetonitrile 40:60, a symmetrical peak with good separation was achieved.
Validation of the method
Validation studies18-19 were carried out on different parameters as per ICH guideline such as recovery study, method precision and intermediate precision, range, LOD and LOQ, System suitability study. Linearity was established at five different concentrations with good correlation coefficient. Accuracy or recovery was conducted at different levels. Precision studies were carried out using the same optimized conditions and RSD20-21 was less than 2 %. LOD and LOQ indicate, the method was highly sensitive and fast. The method was found highly specific since there was no interference from the excipients of the formulations. Analytical solution was found stable up to 24 hrs on refrigeration (Table 1).
Forced degradation studies
Different stress conditions22,23 were used in the study. In case of hydrolytic stress degradation under acidic 0.1N hydrochloric acid was used as reagent whereas in alkaline condition 0.1N sodium hydroxide was incorporated. Drug was refluxed with acid, alkali and water for about 6 hrs at 60°C targeting 5-20% degradation. In oxidative degradation studies 3% v/v hydrogen peroxide was used and kept at room temperature for 15 min. In thermal studies, conducted for 24 hrs at 110°C. In photolytic degradation, drug was exposed to sunlight for 24 hrs. Slightly high degradation was noted for peroxide stress condition than other conditions. Only one degradant peak was observed which did not interfere with the main peak. From the degradation studies, the peak angle of the drug was less than the purity threshold which indicates there was no merging of impurity peak with the analyte peak (Table 2 and Figure 1-5).
|100 % level|
|Linearity Range||5 –25µg/ml|
|System suitability parameters|
|USP Plate count||9097.50|
|USP Tailing factor||0.95333|
|Room Temperature||2 h|
A Rapid, stable and sensitive stability indicating assay method was developed and validated. Extent of degradation increases with an increase in the duration of stress studies and the degradant peak was not interfering with main peak. It can be concluded that the developed method is stable for specified period of time in different stress conditions. This method can be extended for characterization of the degradants with integrated approach and they can be effectively applied for routine analysis in various drug testing departments.