Experience of Introduction of Two Species of Eryngium in the North Caucasus

Aleksandrovna, Mikhailovna, and Alexeevich: Experience of Introduction of Two Species of Eryngium in the North Caucasus

Authors

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INTRODUCTION

The genus Eryngium L. belongs to the family Apiaceae, which consists of about 300 species, species growing in the tropical, subtropical and temperate latitudes of the worldwide.1 Species of Eryngium L. are widespread in Central Asia, America, Central and South-Eastern Europe. In Russia, grows about 20 species, in the Caucasus - 8 species.2 Eryngium caucasicum Trautv. and Eryngium planum L. - perennial herbaceous plants. The aboveground and underground parts of these species contain terpenoids, saponins, flavonoids, phenolic acids, coumarins, polyacetylenes and other biologically active compounds.3-6 These substances determine diuretics, antispasmodic, antifungal, cytotoxic, antitubercular and stimulating properties.7-8 Among the known species of the genus Eryngium, we studied two species: E. caucasicum Trautv. and E. planum L., which are characterized by a wide variety of active substances and use in folk medicine.9 Plants have a large vegetative mass, which is important when harvesting raw materials. Studies on the introduction of these species were carried out from 2014 to 2017. Some observational data for 2014-2016 are published in the press.10-11 In this paper, we propose the results of observations for 2017, taking into account climatic data and general recommendations for the cultivation of two species of the genus Eringium in the Stavropol Territory (the North Caucasus).

MATERIALS AND METHODS

Plains of the Stavropol Territory receive 120-125 kcal / cm2 of solar radiation per year. The maximum temperature reaches 37-45°C. The amount of rainfall is small, 170-180 mm per year. Seeds of Eryngium caucasicum Trautv. and Eryngium planum L. were harvested in various regions of Dagestan, Kabardino-Balkaria and the Stavropol Territory. Mature seeds were collected for research in August-September 2014. Seeds were placed in the soil in early spring (March) and late autumn (November). Seeds are small, 2-3 mm in length, sown to a depth of 1-2 cm, between rows - 50-60 cm, since leaves can reach a diameter of 60 cm.

The main methods of research are: Observation, Field, Mathematical.

RESULTS AND DISCUSSION

Observations were carried out on plants of the first and second years of development. The species are propagated by seeds. In the first year, seeds were sown and the formation of a rosettes of leaves was observed. In 2017, spring seed sowing was carried out on March 30, when the air temperature was 12 degrees Celsius. Seedlings appeared on April 14-16. Rosettes of leaves began to form on May 4-6. The dynamics of rosettes growth, the number of formed leaves and temperature data in the first year of development are presented in Table 1.

Table 1

Duration of vegetation of two species of Eringium in the first year of development.

Date of observationAir temperature, °СEryngium caucasicumEryngium planum
Diameter of rosettes of leaves,
cm
Number of leavesDiameter of rosettes of leaves,
cm
Number of leaves
12.05.17 г.173,5-93-77-93-4
19.05.17 г.1412-178-118-133-5
26.05.17 г.2013-2020-238-143-5
01.06.17 г.2015-2522-2513-184-5
08.06.17 г.2819-2724-2614-195-6
15.06.17 г.2221-2927-3015-206-7
22.06.17 г.2022-3031-3517-206-8
02.07.17 г.3624-3234-4318-207-8
11.07.17 г.2632-3637-4820-287-8
20.07.17 г.2033-3837-4823-327-8
08.09.17 г.2436-3937-4826-357-8

According to the Table, that the formation of rosettes of leaves in the first year of development continues 60-70 days. During August, slightly increased the size of leaf rosettes. At the same time, air temperature has no special effect on the formation of rosettes. The diameter of the rosettes and the number of leaves in the Eryngium planum are somewhat smaller than in the Eryngium caucasicum (Figure 1).

Figure 1

Rosettes of leaves: A - Eryngium caucasicum; B – Eryngium planum.

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In this state, the plants were before winter. In winter, some of the leaves die off, but in spring the new leaves grow back.

In 2017, the growth of plants of the second year began on March 8-9, when the air temperature was 4-9°С. Rosettes of leaves formed in Eryngium caucasicum until April 30, in Eryngium planum - until May 12. Their dimensions were 58-60 cm and 47-54 cm, respectively.

The results of observations on the phases of plant development in the second year of vegetation are presented in Table 2.

Table 2

Dynamics of shoots growth of two species of Eryngium.

Date of observationAir temperature, °СEryngium caucasicumEryngium planum
Height of shoots, cmDevelopment phaseHeight of shoots, cmDevelopment phase
06.05.17 г.85-15growth of shootsrosette of leaves
08.05.17 г.2025-40growth of shootsrosette of leaves
12.05.17 г.1735-49growth of shootsrosette of leaves
16.05.17 г.1048-67growth of shoots7-22growth of shoots
21.05.17 г.2350-90growth of shoots18-35growth of shoots
25.05.17 г.1451-96growth of shoots27-47growth of shoots
28.05.17 г.1860-98growth of shoots40-62beginning of budding
01.06.17 г.2080-105beginning of budding40-63budding
08.06.17 г.2894-125budding74-87budding
20.06.17 г.20112-134budding93-109budding
02.07.17 г.36135-140beginning of flowering100-115beginning of flowering
11.07.17 г.26135-140flowering100-115flowering
16.07.17 г.27135-140flowering116-120flowering
30.07.1725135-140flowering116-120end of flowering
10.08.17 г.29135-140end of flowering116-120beginning of fruits ripening
18.08.17 г.27135-140beginning of fruits ripening116-120fruits ripening
08.09.17 г.24135-140fruits ripening

Plants in the second year of vegetation first form rosettes of leaves, then begin to develop shoots. The phase of budding is shown in Figure 2.

Figure 2

Plants in the budding phase: А – Eryngium caucasicum; B – Eryngium planum.

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The duration of vegetation phases is presented in Table 3.

Table 3

Duration of vegetation phases of two species of Eringium in the second year of development (days).

SpeciesRosettes of leavesGrowth of shootsBuddingFloweringFruits ripeningTotal
Eryngium caucasicum5025-3025-304020-30160-180
Eryngium planum6510-1515-2020-2510-15120-140

Such differences in the duration of developmental phases are explained by the different degree of branching of shoots. In Eryngium planum inflorescences less branched, more compact, Eryngium caucasicum forms a large number of lateral branches - the axes of inflorescences.

As a result of the study, it was found that when sowing seeds at the end of March it took 150-160 days to completely form a rosette of leaves in the first year of vegetation. Rosettes of leaves had a diameter of 35-39 cm. In the second year of vegetation, the formation of new leaves and their growth began on March 8-9 at a temperature of 4-9°C. The fruits of Eryngium planum ripened in August, and Eryngium caucasicum - in September. For the full development of plants, it took 120-140 and 160-180 days, respectively. Care of plants consisted of weeding from weeds and watering. In the absence of precipitation it is necessary to water 1-2 times a week. The spring-summer period of 2017 was characterized by a large amount of rainfall in April-June. Therefore, the plants of this year have grown to 120-140 cm.

Observations in 2017 confirm the possibility of successful cultivation of two species of Eringium in the Stavropol Territory.

CONCLUSION

Thus, as a result of observations of the vegetation of two species of Eringium in the Stavropol Territory (the North Caucasus) during 2014-2017, we propose the following recommendations for their introduction:

  1. Plants must be propagated by seeds.

  2. Seed sowing should be done in late February - early March. In addition, you can sow in the autumn - in late October - early November.

  3. Seeds are placed in the soil at a depth of 1-2 cm, between rows - 50-60 cm.

  4. Make weeding and watering as necessary.

  5. Chemical protection of plants from pests is not required.

  6. Harvesting fruits and seeds in August-September.

ACKNOWLEDGEMENT

This research project was carried out with the assistance of the Fund for the development of research. The authors are grateful to the head of Pyatigorsk Medical and Pharmaceutical Institute V. L. Adgienko.

CONFLICT OF INTEREST

When performing this research conflicts of interest did not arise, since all of the authors have conducted research work comprehensively.

REFERENCES

1 

Muckensturm B, Boulanger A, Farahi M, Reduron JP , authors. Secondary metabolites from Eryngium species. Natural product research. 2010; 24 (5): 391 –7

2 

Czerepanov SK , author. Vascular plants of Russia and adjacent states (the former USSR). Cambridge University Press. 2007. p. 992

3 

Erdem SA, Nabavi SF, Orhan IE, Daglia M, Izadi M, Nabavi SM , authors. Blessings in disguise: a review of phytochemical composition and antimicrobial activity of plants belonging to the genus Eryngium. DARU Journal of Pharmaceutical Sciences. 2015; 23 (1): 53

4 

Conea S, Vlase L, Chirila I , authors. Comparative study on the polyphenols and pectin of three Eryngium species and their antimicrobial activity. Cellulose chemistry and technology. 2016; 50 (3-4): 473 –81

5 

Riekandeh SM, Mazandarani M, Ebrahimzadeh MA, Zargari M , authors. Antioxidant activities of Eryngium caucasicum inflorescence. European review for medical and pharmacological sciences. 2016; 20 (5): 946 –9

6 

Hashemabadi D, Kaviani B , authors. Chemical constituents of essential oils extracted from the leaves and stems of Eryngium caucasicum Trautv. from Iran. Journal of Essential Oil Bearing Plants. 2011; 14 (6): 693 –8

7 

Nasuhova AM, Orobinskaya VN, Konovalov DA, Kuzmina U, Shchanukova EA , authors. Natural polyacetylene compounds with antitubercular activity. Medical news of the North Caucasus. 2016; 11 (4): 595 –9

8 

Spiridonov NA, Konovalov DA, Arkhipov VV , authors. Cytotoxicity of some Russian ethnomedicinal plants and plant compounds. Phytotherapy Research. 2005; 19 (5): 428 –32

9 

Ebrahimzadeh MA, Chitsaz Z, Shokrzadeh M, Ataie A, Ataee R , authors. et al. Evaluation of Anticonvulsant Activities of Eryngium caucasicum with Maximal Electroshock and Kindling Model of Seizure in Mice. Iran J Psychiatry Behav Sci. 2017; 11 (1): e3571

10 

Eliseeva LM, Scherbakova EA, Konovalov DA, Galkin MA , authors. Active parts and seed efficiency of Eryngium planum from Apiaceae family. Pharmacy and pharmacology. 2015; 1 (8): 14 –6

11 

Shcherbakova EA, Konovalov DA, Eliseeva LM , authors. Possibility of Eryngium caucasicum and Eryngium planum introduction in the region of Mineralnye Vody, Caucasus. Aspirantskiy Vestnik Povolzhiya. 2017; 1-2: 221 –7

GRAPHICAL ABSTRACT

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SUMMARY

  • The article gives recommendations on the cultivation of Eryngium caucasicum Trautv. and Eryngium planum L. in the Caucasus. Seeds should be placed in the ground in late autumn (late October-early November) or early spring (late February-early March). Seeds are sown to a depth of 1-2 cm, between rows of 50-60 cm. The seedlings appear on the 14-16th day. In the first year of vegetation, a rosette of leaves is formed. By the beginning of September, the rosettes of leaves have a diameter of 35-39 cm. The plants of the second year are Eryngium caucasicum Trautv. Develop within 180 days and Eryngium planum L. - for 160 days. Care of plants is the removal of weeds and watering. Collection of seeds and fruits is recommended in August-September.

ABOUT AUTHORS

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Shcherbakova Ekaterina Aleksandrovna: Postgraduate student at the Chair of Pharmacognosy, Department of Pharmacognosy and Botany Pyatigorsk medical-pharmaceutical Institute – branch FGBOU VO VolgGMU Ministry of health of Russia, RUSSIA. Research interests: Phytochemistry, Pharmacognosy.

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Eliseeva Lyudmila Mikhailovna: Candidate of Pharmaceutical Sciences, Senior Lecturer, Department of Pharmacognosy and Botany Pyatigorsk Medical-Pharmaceutical Institute – branch FGBOU VO VolgGMU Ministry of health of Russia, RUSSIA. Research interests: Study of the flora of the Caucasian Mineral Waters, Micro morphologic study of plants of the Caucasus.

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Konovalov Dmitryi Alexeevich: Doctor of Pharmaceutical Sciences, Professor, Department of Pharmacognosy and Botany Pyatigorsk, Medical-Pharmaceutical Institute – branch FGBOU VO VolgGMU Ministry of health of Russia, RUSSIA. Research interests: Phytochemistry, Pharmacognosy, Sesquiterpene lactones, Polyacetylene compounds.